Host intestinal biomarker identification in a gut leakage model in broilers

Table 5 Proteins of which the normalized abundance in ileal content was significantly different between control and challenged groups

Accession number	Protein name	Abbreviation	p-value	Highest mean
O57579	Aminopeptidase Ey	AMPN	0.0012	Control
Q10751	Angiotensin-converting enzyme	ACE	0.0006	Control
P08250	Apolipoprotein A-1	APOA1	0.0364	Challenge
P00508	Aspartate aminotransferase, mitochondrial	AATM	0.0067	Control
Q05744	Cathepsin D	CATD	0.0203	Control
P02263	Histone H2A-IV	H2A4	0.0079	Challenge
P20763	Ig lambda chain C region	LAC	0.0155	Control
P04210	Ig lambda chain V-1 region	LV1	0.0370	Control
P41263	Retinol-binding protein 4	RET4	0.0399	Challenge
P80566	Superoxide dismutase [Cu–Zn]	SOD	0.0004	Control
P27731	Transthyretin	TTR	0.0091	Control
O93277	WD repeat-containing protein 1	WDR1	0.0027	Control

Proteomics using high performance liquid chromatography–mass spectrometry (HPLC–MS) was performed on ileal content of animals from the control (n = 9) and challenge (n = 9) group at day 26. This resulted in 3 proteins with a significantly higher normalized abundance (p < 0.05) and 9 proteins with a significantly lower normalized abundance (p < 0.05) in challenged birds.

ISSN: 1297-9716