Host intestinal biomarker identification in a gut leakage model in broilers

Table 4 Proteins of which the normalized abundance in colonic content was significantly different between control and challenged groups

Accession number	Protein name	Abbreviation	p-value	Highest mean
Q90734	Alpha-actinin-4	ACTN4	0.0385	Challenge
P17153	Annexin A5	ANXA5	0.0266	Challenge
P08250	Apolipoprotein A-1	APOA1	0.0277	Challenge
P11722	Fibronectin	FINC	0.0106	Challenge
P02112	Hemoglobin subunit beta	HBB	0.0158	Challenge
P08940	Myeloid protein 1	MIM1	0.0008	Challenge
P16039	Nucleophosmin	NPM	0.0071	Challenge
P10184	Ovoinhibitor	IOV7	0.0254	Challenge
P80566	Superoxide dismutase [Cu–Zn]	SOD	0.0287	Control
P27731	Transthyretin	TTR	0.0317	Challenge

Proteomics using high performance liquid chromatography–mass spectrometry (HPLC–MS) was performed on colonic content of animals from the control (n = 9) and challenged (n = 9) group at day 26. This resulted in 9 proteins with a significantly higher normalize abundance (p < 0.05) and 1 protein with a significantly lower normalized abundance (p < 0.05) in challenged birds.

ISSN: 1297-9716