Figure 3

Cloning, expression and purification of HcGST HcTTR and HcCab. A The amplification of HcGST, HcTTR and HcCab. 0.1% agarose gel electrophoresis was used to detect amplification of PCR products. M: DNA standard molecular weight DL2000; Lane 1: the amplification of HcGST; Lane 2: the amplification of HcTTR; Lane 3: the amplification of HcCab; B The purification of rHcGST, rHcTTR and rHcCab. Protein samples were resolved by SDS-PAGE on 12% polyacrylamide gel and stained with Coomassie brilliant blue R250. M: Standard protein molecular marker; Lane 1: soluble extract of cultured cells for rHcGST; Lane 2: purified recombinant HcGST was approximately 41.2 kDa; Lane 3: soluble extract of cultured cells for rHcTTR; Lane 4: purified recombinant HcTTR was approximately 35.4 kDa; Lane 5: soluble extract of cultured cells for rHcCab; Lane 6: purified recombinant rHcCab was approximately 34.1 kDa.