Figure 1

Acquisition of HcESPs, rIL4 and the immunoprecipitates. A The collection of HcESPs. M: standard protein molecular marker; Lane 1: HcESPs were resolved by SDS-PAGE on 12% polyacrylamide gel and stained with Coomassie brilliant blue R250. B The purification of rIL4. Protein samples were resolved by SDS-PAGE on 12% polyacrylamide gel and stained with Coomassie brilliant blue R250. M: standard protein molecular marker; Lane 1: soluble extract of cultured cells for rIL4; Lane 2: purified recombinant IL4 was approximately 30.7 kDa. C Western blot analysis of the interaction between HcESPs and rIL4 in vitro. Lane M: protein marker (ordinate values in kDa); Lane 1: the immunoprecipitates (rIL4 and HcESPs) obtained by Co-IP with IgG HcESPs; Lane 2: rat normal IgG was used as a control sample. The results demonstrated that HcESPs could bind to rIL4 in vitro.