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Table 3 qPCR verification of differentially expressed genes in the mutant strain Yb2Δ gldM and complementation strain cYb2Δ gldM

From: Riemerella anatipestifer GldM is required for bacterial gliding motility, protein secretion, and virulence

Gene locusa Description of genes 2−ΔΔCt
Yb2ΔgldM cYb2ΔgldM
AS87_RS00230 b PorT protein 3.5925 1.472
AS87_RS02915 Hypothetical protein 2.445 1.3755
AS87_RS09140 Hypothetical protein 2.0875 1.2058
AS87_RS09480 Twitching motility protein PilT 2.06 1.2397
AS87_RS02755 Hypothetical protein 2.0533 0.9432
AS87_RS08665 Tat pathway signal sequence domain-containing protein 2.03 1.3947
AS87_RS09585 Hypothetical protein 1.6533 1.1028
AS87_RS08925 SusC/RagA family TonB-liked outer membrane protein 1.4225 1.6531
AS87_RS08050 Carbohydrate-bonding protein 1.3875 0.8593
AS87_RS04485 Cbb3-type cytochrome oxidase component FixQ 1.35 1.021
AS87_RS07185 Hypothetical protein 1.28 1.0352
AS87_RS09360 Hypothetical protein 1.2725 1.1408
AS87_RS05780 Hypothetical protein 1.12 0.8526
AS87_RS05060 Hypothetical protein 1.0733 0.7474
AS87_RS10475 Hypothetical protein 1.0233 0.8745
AS87_RS08045 RagA family TonB-liked outer membrane protein 0.985 1.2256
AS87_RS00555 RNA polymerase sigma24 factor 0.98 1.0867
AS87_RS08885 Hypothetical protein 0.947 1.591
AS87_RS09380 Hypothetical protein 0.945 1.0497
AS87_RS01535 Hypothetical protein 0.93 1.2226
AS87_RS05020 Hypothetical protein 0.9175 0.895
AS87_RS09995 Hypothetical protein 0.8825 1.4948
AS87_RS08585 ABC transporter 0.8425 1.5157
AS87_RS00880 ABC transporter related protein 0.6175 0.9659
AS87_RS08470 b Gliding motility protein GldN 0.615 0.7813
AS87_RS07850 Hypothetical protein 0.47 0.9075
AS87_RS01360 Hemolysin D 0.34 0.5586
AS87_RS01365 Hypothetical protein 0.33 0.8645
AS87_RS01350 Heavy metal efflux pump, CzcA family 0.295 0.7846
AS87_RS01355 Transporter 0.225 0.6925
  1. aBased on the R. anatipestifer Yb2 genome (accession number: CP007204).
  2. bThese genes encode T9SS components.