Riemerella anatipestifer GldM is required for bacterial gliding motility, protein secretion, and virulence

Table 2 Predicted R. anatipestifer Yb2 proteins secreted by T9SS identified with LC–MS/MS

Locus tag	Molecular mass (KD^a)	T9SS CTD^b	Proteins	Predicted protein function	Relative change of protein^c	Relative change of protein^d
AS87_RS03090	13.49	+	Uncharacterized protein	Unknown	6.0762	1.6765
AS87_RS06600	117.29	+	Pkd domain containing protein	Collagenolytic of the catalytic	0.1004	1.1848
AS87_RS07295	93.945	+	Fibronectin type iii domain protein	Serine-type endopeptidase activity	0.0274	0.9055
AS87_RS02625	39.812	+	Endonuclease i	Endonuclease activity	0.2592	0.7272
AS87_RS00980	68.225	+	Metallophosphoesterase (MPPE)	Acid phosphatase activity	0.0434	1.3085
AS87_RS04190	77.52	+	Subtilisin-like serine protease	Serine-type endopeptidase activity	0.2041	1.1190
AS87_RS03200	117.12	+	Uncharacterized protein	Unknown	0.0063	1.2016
AS87_RS02020	83.131	+	Immunoreactive 84 kDa antigen pg93	Metal ion binding	0.3273	1.1742
AS87_RS00835	161.5	+	Peptidase s8 and s53 subtilisin kexinsedolisin	Serine-type endopeptidase activity	0.0045	1.2558

^aProteins in cell-free culture fluid from the wild-type strain Yb2, mutant strain Yb2ΔgldM and complementation strain cYb2ΔgldM were analyzed with LC–MS/MS.
^bT9SS CTD identified with BLASTP analysis. + Indicates that the protein has a CTD.
^cRelative difference in protein secretion between Yb2ΔgldM and Yb2.
^dRelative difference in protein secretion between cYb2ΔgldM and Yb2.

ISSN: 1297-9716