Figure 1

Identification of the mutant strain Yb2ΔgldM and complementation strain cYb2ΔgldM. A PCR amplification. M: DL2000 DNA Marker (Vazyme, Nanjing, China). Lane 1: the R. anatipestifer gldM gene and 16S rRNA were amplified from the wild-type strain Yb2 with the primer pairs AS87_RS08465-F/AS87_RS08465-R and RA16S rRNA-F/RA 16S rRNA-R, respectively, generating a 1560-bp fragment of gldM and a 792-bp fragment of R. anatipestifer 16S rRNA, respectively. Lane 2: a 792-bp fragment of RA 16S rRNA was amplified with the primer pair RA16S rRNA-F/RA 16S rRNA-R, but no 1560-bp fragment of the RA gldM gene was amplified with the primer pair AS87_RS08465-F/AS87_RS08465-R from the mutant strain Yb2ΔgldM. Lane 3: both the R. anatipestifer gldM gene and 16S rRNA were amplified from the complementation strain cYb2ΔgldM with the primer pairs AS87_RS08465-F/AS87_RS08465-R and RA16S rRNA-F/RA 16S rRNA-R, respectively, generating a 1560-bp fragment of gldM or a 792-bp fragment of RA 16S rRNA, respectively. Lane 4: distilled water was used as a non-strain control. B qPCR analyses of the polar effect in the mutant strain Yb2ΔgldM. No polar effect was observed. C Bacterial growth curves: no significant differences were detected among the R. anatipestifer wild-type strain Yb2, mutant strain Yb2ΔgldM, and complementation strain cYb2ΔgldM.