Table 1 Bacterial strains and plasmids used in this study
Strains or plasmids | Characteristics | References |
|---|---|---|
Strains | ||
 DE719 | O2:K1 | [14] |
 ΔcpxR | cpxR gene deletion mutant in DE719 | This study |
 CΔcpxR | ΔcpxR with plasmid pSTV28-cpxR | This study |
 DH5α-pRCL | DH5α with plasmid pRCL | This study |
 DH5α-pRCL-Phcp2B | DH5α with plasmid pRCL-Phcp2B | This study |
 DE719-pBAD | DE719 with plasmid pBAD/Myc-His | This study |
 DE719-pnlpE | DE719 with plasmid pBAD-nlpE | This study |
 ΔcpxR-pBAD | ΔcpxR with plasmid pBAD/Myc-His | This study |
 ΔcpxR-pnlpE | ΔcpxR with plasmid pBAD-nlpE | This study |
 DH5α | F-, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ- | TIANGEN |
 BL21 (DE3) | F-, ompT, hsdS (r − B m − B ) gal, dcm (DE3) | TIANGEN |
Plasmids | ||
 pET28a(+) | Kan, F1 origin, His tag | Novagen |
 pET28a-cpxR | pET28a(+) carrying cpxR gene | This study |
 pSTV28 | Cm, p15A origin | Takara |
 pSTV28-cpxR | pSTV28 derivative harboring cpxR gene | This study |
 pBAD/Myc-His | Amp, ColE1 derivative cloning vector, pBAD (ara) promoter | Invitrogen |
 pBAD-nlpE | pBAD/Myc-His expressing E. coli K12 NlpE-His from the pBAD (ara) promoter | This study |
 pRCL | Cm, promoterless lacZ, | This study |
 pRCL-Phcp2B | pRCL harboring hcp2B promoter | This study |
 pKD46 | Amp, expresses λ red recombinase | [45] |
 pKD3 | Cm, template plasmid | [45] |
 pCP20 | Cm, Amp, yeast Flp recombinase gene, FLP | [45] |