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Table 1 Bacterial strains and plasmids used in this study

From: The CpxR regulates type VI secretion system 2 expression and facilitates the interbacterial competition activity and virulence of avian pathogenic Escherichia coli

Strains or plasmids Characteristics References
Strains
 DE719 O2:K1 [14]
 ΔcpxR cpxR gene deletion mutant in DE719 This study
 CΔcpxR ΔcpxR with plasmid pSTV28-cpxR This study
 DH5α-pRCL DH5α with plasmid pRCL This study
 DH5α-pRCL-Phcp2B DH5α with plasmid pRCL-Phcp2B This study
 DE719-pBAD DE719 with plasmid pBAD/Myc-His This study
 DE719-pnlpE DE719 with plasmid pBAD-nlpE This study
 ΔcpxR-pBAD ΔcpxR with plasmid pBAD/Myc-His This study
 ΔcpxR-pnlpE ΔcpxR with plasmid pBAD-nlpE This study
 DH5α F-, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17(rk, mk+), phoA, supE44, λ- TIANGEN
 BL21 (DE3) F-, ompT, hsdS (r B m B ) gal, dcm (DE3) TIANGEN
Plasmids
 pET28a(+) Kan, F1 origin, His tag Novagen
 pET28a-cpxR pET28a(+) carrying cpxR gene This study
 pSTV28 Cm, p15A origin Takara
 pSTV28-cpxR pSTV28 derivative harboring cpxR gene This study
 pBAD/Myc-His Amp, ColE1 derivative cloning vector, pBAD (ara) promoter Invitrogen
 pBAD-nlpE pBAD/Myc-His expressing E. coli K12 NlpE-His from the pBAD (ara) promoter This study
 pRCL Cm, promoterless lacZ, This study
 pRCL-Phcp2B pRCL harboring hcp2B promoter This study
 pKD46 Amp, expresses λ red recombinase [45]
 pKD3 Cm, template plasmid [45]
 pCP20 Cm, Amp, yeast Flp recombinase gene, FLP [45]