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Table 2 List of adjusted primers and probes used for the RT-PCR multiplex for subtyping

From: Longitudinal field studies reveal early infection and persistence of influenza A virus in piglets despite the presence of maternally derived antibodies

Assay Primer/Probe Sequence (5′-3′)
H1pdm H1fw2sw-2 GAA GTT CAA GCC GGA AAT AGC A
H1av H1-av-P ROX-TCT GGT TAC GCA GCW GAT CAG AAA AGC AC-BHQ2
H3hu H3-hu_mink-F GAT GAT GGA GAA AAC TGC ACA CTA
N2sw N2-F GAG TAT GGT GGA CBT CAA AYA G
N2-R TTG CGA AAG CTT ATA TAG GCA TGA
N2-P AF532-CCA TCA GGC CAT GAG CCT GAV CCA TA-BHQ1
N2hu N2hu-P AF532-T[+C]A [+A]CT CYA CAT AAA AGC ACC [+G]-BHQ1
  1. “F” indicates the forward primer, “R” indicates the reverse primer, and “P” indicates the probe. Letters in “[ ]” indicate a locked nucleic acid (LNA). The letters in italics indicate the reporter and quencher.