Longitudinal field studies reveal early infection and persistence of influenza A virus in piglets despite the presence of maternally derived antibodies

Table 2 List of adjusted primers and probes used for the RT-PCR multiplex for subtyping

Assay	Primer/Probe	Sequence (5′-3′)
H1pdm	H1fw2sw-2	GAA GTT CAA GCC GGA AAT AGC A
H1av	H1-av-P	ROX-TCT GGT TAC GCA GCW GAT CAG AAA AGC AC-BHQ2
H3hu	H3-hu_mink-F	GAT GAT GGA GAA AAC TGC ACA CTA
N2sw	N2-F	GAG TAT GGT GGA CBT CAA AYA G
	N2-R	TTG CGA AAG CTT ATA TAG GCA TGA
	N2-P	AF532-CCA TCA GGC CAT GAG CCT GAV CCA TA-BHQ1
N2hu	N2hu-P	AF532-T[+C]A [+A]CT CYA CAT AAA AGC ACC [+G]-BHQ1

“F” indicates the forward primer, “R” indicates the reverse primer, and “P” indicates the probe. Letters in “[ ]” indicate a locked nucleic acid (LNA). The letters in italics indicate the reporter and quencher.