Figure 1

The transcription levels of acs, yjcH, actP, ackA, pta, and satP genes in APEC/ExPEC strains. qRT-PCR of these genes was determined relative to the transcription level of gene dnaE. Significant differences in qRT-PCR data were identified using one-way ANOVA statistical analysis (***P < 0.01). A The transcriptional differences of acs, yjcH, actP, ackA, pta, and satP genes during FY26 survival in HD11 macrophages relative to that in routine non-infectious condition at 41 °C in vitro. qRT-PCR data (uninfected FY26 in vitro; infected FY26 at 4 hpi or 8 hpi) acquired from three individual experiments were used to measure the differences (fold-change) of these genes transcription. B The transcriptional differences of acs, yjcH, actP, ackA, pta, and satP genes during FY26 survival in RAW264.7 macrophages relative to that in routine non-infectious condition at 37 °C in vitro. qRT-PCR data (uninfected FY26 in vitro; infected FY26 at 4 hpi or 8 hpi) was acquired from three individual experiments. C The transcription levels of ackA, pta, and satP genes between FY26 invasion in non-phagocytic cells (DF-1 or HEp2) relative to the cultured at 37 °C in vitro. qRT-PCR data (uninfected FY26 in vitro, infected FY26 in DF-1 cells at 4 hpi, and infected FY26 in HEp2 at 4 hpi) from three individual experiments were used to measure the differences (fold-change) of these genes transcription. D Transcriptional differences of acs, yjcH, actP, ackA, pta, and satP genes during strain CVCC249 survival in HD11 and RAW264.7 macrophages at 4 hpi relative to the routine condition in vitro. E Transcriptional differences of acs, yjcH, actP, ackA, pta, and satP genes during RS218 survival in HD11 and RAW264.7 macrophages at 4 hpi relative to the routine condition in vitro.