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Table 2 Primer sequences for real-time quantitative PCR

From: UBXN1 interacts with the S1 protein of transmissible gastroenteritis coronavirus and plays a role in viral replication

Gene

Accession number

Sequence (5′–3′)

Size (bp)

Concentration

β-Actin

XM_003124280.2

F: CTCTTCCAGCCCTCCTTCC

97

0.5 µM

R: GGTCCTTGCGGATGTCG

UBXN1

XM_003353824.1

F: TTGGAGCTTGTGGCCCAGAA

139

R: GGCGCATTTCGTCTTCCTGG

TGEV-N

NC_038861.1

F: TTCAACCCCATAACCCTCCAACAA

136

R: GGCCCTTCACCATGCGATAGC

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Veterinary Research

ISSN: 1297-9716