Interference and overexpression of UBXN1. A, B siRNA interference fragments reduced the expression of UBXN1. The relative expression of UBXN1 was assessed by Western blotting after 24 h of interference. Control: normal group; NC1: transfection reagent group; siRNA: fragment 1. B The lentiviral plasmid overexpressed UBXN1. The plasmid was transfected into cells for 36 h, and the relative expression of UBXN1 was assessed by Western blotting. Control: normal group; NC2: transfection reagent group; Overexpression: transfection plasmid group. C Molecular modulators of UBXN1 do not affect cell viability. Cell viability was determined by an MTT assay after treatment with the interference fragment (SiRNA) or overexpression plasmid (Plenti-CMV-GFP-2A-Puro-UBXN1) for 48 h. The data are the means ± SEMs of three independent experiments (t-test, #p > 0.05). Control: normal group; IF1: interference fragment 1 group; TP: transfection plasmid group.