Figure 4
From: Newcastle disease virus selectively infects dividing cells and promotes viral proliferation
NDV selectively infects HeLa cells. A The presence of sialic acid was detected in HeLa and BHK cells by FACS analysis; unstained cells were used as a blank control. B Fluorescence microscopy images of NDV (GFP+) cells treated with different viral titers of NDV. Scale bars represent 50 μm. C Schematic of the coculture assay. D Fluorescence microscopy images of NDV (GFP+) and RFP in cocultured cells; the arrow indicates an NDV-infected BHK-RFP cell. Scale bars represent 50 μm. E Flow cytometry was used to analyze RFP and GFP expression in BHK-RFP/HeLa and BHK-RFP/BHK cell cocultures. Numbers reflect the percentage of cells. Data are presented as the mean ± SD of triplicate samples from three independent experiments. **P < 0.01 by t-test. F Schematic of the coculture assay. G Fluorescence microscopy images of NDV (GFP) and RFP in cocultured cells; the arrow indicates an NDV-infected HeLa-RFP cell. Scale bars represent 50 μm. H Flow cytometry was used to analyze RFP and GFP expression in HeLa-RFP/HeLa and HeLa-RFP/BHK cell cocultures. Numbers reflect the percentage of cells. Data are presented as the mean ± SD of triplicate samples from three independent experiments. *P < 0.05 by t-test.