Figure 3
From: Newcastle disease virus selectively infects dividing cells and promotes viral proliferation
NDV selectively infects HeLa cells but not neuraminidase-treated HeLa cells. A Morphologic observation of untreated and neuraminidase-treated HeLa cells. B The presence of sialic acid was detected in neuraminidase-treated HeLa cells by FACS analysis; cells treated without neuraminidase were used as controls. Data are presented as the mean ± SD of triplicate samples from three independent experiments. ***P < 0.001 by t-test. C Schematic of the coculture assay. D Fluorescence microscopy images of NDV (GFP) and RFP expression in cocultured cells. Scale bars represent 50 μm. E Flow cytometry analysis of RFP and GFP expression in HeLa-RFP/HeLa and HeLa-RFP/neuraminidase-treated HeLa cells. Numbers indicate the percentage of cells. n = 3 per group of four analyses. Data are presented as the mean ± SD of triplicate samples from three independent experiments. **P < 0.01 by t-test.