Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates

Table 1 Primer sequences and amplicon details used in RT-qPCR assay for Flavobacterium columnare

Primer name	Sequence (5′–3′)	Amplicon length (bp)	Efficiency (%)	R² (%)	References
Primers for target genes (gld-genes are involved in motility, spr-genes in adhesion and porV in virulence)
FC_gldK_fwd	GCCAAATAGCACCCTCTATCA	102	103.0	98.9	This study
FC_gldK_rev	AACAGAAGCCGAATGGGAATA	102	103.0	98.9	This study
FC_gldL_fwd	GCTTCTGTACCTAAACCAGCA	94	99.9	99.5	This study
FC_gldL_rev	TTGGTGCGGCAGTAGTAATC	94	99.9	99.5	This study
FC_gldM_fwd	ACTACCACATCACCTCTTTGTG	93	102.4	99.2	This study
FC_gldM_rev	TCCAGGGCAACCAACAATAG	93	102.4	99.2	This study
FC_gldN_fwd	GCAAGCGCTATGCTTATTGCTGGT	131	97.8	99.0	[31]
FC_gldN_rev	GCAGTTGGTTGTCCCCCTGCT	131	97.8	99.0	[31]
FC_sprA_fwd	AGGCGATGGTATTTCGTTAGG	122	101.9	99.5	This study
FC_sprA_rev	GTACGCGTCCTGCTTGATAA	122	101.9	99.5	This study
FC_sprE_fwd	AGCCGTGCAGAAGATAAAGC	151	92.4	99.0	[31]
FC_sprE_rev	ACGCTTCTAATGCGGGTACAA	151	92.4	99.0	[31]
FC_sprT_fwd	AACCAGGACTGCATTACGGA	144	91.1	99.2	[31]
FC_sprT_rev	GCTTGATGTTACCTGTGCGTT	144	91.1	99.2	[31]
FC_porV_fwd	GTGCCAACTCCTAAAACAGCC	152	102.7	99.3	[31]
FC_porV_rev	AAACCTCCTGGAGCATCACC	152	102.7	99.3	[31]
Primers for reference genes
FC_16S_fwd	ACGATCAAACGGCCATTG	119	105.0	99.4	[30]
FC_16S_rev	AGTAACCTGCCTTCGCAATC	119	105.0	99.4	[30]
FC_gap1_fwd	ACCATCCCAAACAGGAGCCGC	98	103.3	99.3	[30]
FC_gap1_rev	CGTCTGCTGTAGGTACGCGCA	98	103.3	99.3	[30]
FC_glyA_fwd	CCAAACCCTTGGGGCTATACAACCC	98	103.2	99.6	[30]
FC_glyA_rev	AGAGGGCCTCCTTGATTACCTGGAA	98	103.2	99.6	[30]
FC_rplQ_fwd	AGCTGCTAAAGTAGGTGACCGTCC	75	96.7	99.1	[30]
FC_rplQ_rev	GCGTTATCTCCTAAACGGTTCCCCA	75	96.7	99.1	[30]

Amplicon length, efficiency, and R² are given for a primer pair consisting of a forward (fwd) and a reverse (rev) primer.

ISSN: 1297-9716