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Figure 6 | Veterinary Research

Figure 6

From: Nuclear localization of Newcastle disease virus matrix protein promotes virus replication by affecting viral RNA synthesis and transcription and inhibiting host cell transcription

Figure 6

Effect of PROX1 knockdown on the viral RNA synthesis and viral replication. The expression levels of PROX1 protein in BSR-T7/5 cells infected with rSS1GFP and rSS1GFP-M/NLSm (A) or transfected with pCI-M and pCI-M/NLSm (B) were examined by Western blotting. The relative levels of the PROX1 protein were compared with the control GAPDH expression. C Effect of the PROX1 siRNA or control siRNA on the expression of endogenous PROX1 in BSR-T7/5 cells. D PROX1 siRNA- or control siRNA-treated BSR-T7/5 cells were infected with rSS1GFP and rSS1GFP-M/NLSm, and viral RNA synthesis corresponding to the NP and P genes were detected by qRT-PCR. E The growth kinetics of rSS1GFP and rSS1GFP-M/NLSm were compared using multicycle growth curves in PROX1 siRNA- or control siRNA-treated cells. Error bars represent standard deviations (mean ± SD) (*P < 0.05; **P < 0.01; ***P < 0.001 compared to the value of rSS1GFP-M/NLSm).

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