Figure 2From: Nuclear localization of Newcastle disease virus matrix protein promotes virus replication by affecting viral RNA synthesis and transcription and inhibiting host cell transcriptionQuantitative analysis of genomic RNA, antigenomic RNA, mRNA and protein in a minigenomic assay. Relative fold expression of genomic RNA (A), antigenomic RNA (B), and mRNA (C) in the minigenome system caused by M or M/NLSm were measured by qRT-PCR. Error bars represent standard deviations (mean ± SD) (*P < 0.05; **P < 0.01; ***P < 0.001 compared to the value of negative control). D Expression of GFP in the minigenome system caused by M or M/NLSm was detected by Western blotting at 36 and 48 hpt. The relative levels of the M and GFP proteins were compared with the control GAPDH expression.Back to article page