Overexpression of K60, IRG1, OASL and CH25H could inhibit ALV-J replication. MDM cells were transfected with pCMV-K60, pCMV-IRG1, pCMV-OASL, pCMV-CH25H and infected with ALV-J strain SCAU-HN06 at 24 post-transfection. MDM cells transfected with pCMV-EGFP as a control. A SCAU-HN06 strain envelop protein was measured by Western blot at 3 hpi. B The level of SCAU-HN06 strain envelop protein was analyzed by Image Studio Ver 5.2 (Odyssey Fc). C The expression of SCAU-HN06 env gene was measured by qPCR at 3 hpi. *p < 0.05, **p < 0.01.