Table 1 Primers used for qPCR and PCR amplification
Types | Name | Sense (5′–3′) | Antisense (5′–3′) |
|---|---|---|---|
siRNA | Epsilon-29 | GCUGAGCGAUACGACGAAATT | UUUCGUCGUAUCGCUCAGCTT |
Epsilon-159 | GGAGAAUAAUCAGCAGCAUTT | AUGCUGCUGAUUAUUCUCCTT | |
Epsilon-643 | GCAGUUGUUACGUGAUAAUTT | AUUAUCACGUAACAACUGCTT | |
Beta-21 | GCUGGUACAGAAAGCCAAATT | UUUGGCUUUCUGUACCAGCTT | |
Beta-138 | CUCUGUUGCCUACAAGAAUTT | AUUCUUGUAGGCAACAGAGTT | |
N.C | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT | |
qPCR primers | 14-3-3β | TGAGAAGAAGCAGCAGATG | TTCCGATGTCCACAGAGT |
14-3-3ε | CGACGAAATGGTGGAGTC | TGCTGGAATGAGGTGTTT | |
PRRSV N gene | AGATCATCGCCCAACAAAAC | GACACAATTGCCGCTCACTA | |
GAPDH (swine) | ACTCACTCTTCCACTTTTGATGCT | TGTTGCTGTAGCCAAATTCA | |
GAPDH (monkey) | ACCCACTCTTCCACCTTCGACGCT | TGTTGCTGTAGCCAAATTCG |