Figure 1

Identification of interactions between 14-3-3 subtypes and NSP2 by confocal microscopy (A and C) and IP (B). A Co-localization of NSP2 with 14-3-3 subtypes, confirmed by immunofluorescence microscopy. Co-localization of EGFP–NSP2 (green) with 14-3-3 β, ε, γ, and τ/ζ (red) was visualized in transfected 293T cells. B 293T cells were transfected with GFP–nsp2 and an empty vector. Proteins associated with NSP2 were pulled down using GFP-Trap and analyzed by Western blot using specific antibodies against 14-3-3β, ε, γ, and τ/ζ. C Co-localization of HP-PRRSV with 14-3-3 subtypes, confirmed by immunofluorescence microscopy. Co-localization of NSP2 of TA-12 (green) with 14-3-3 α/β, ε, γ, and τ/ζ (red) was visualized in Marc-145 cells. Co-localization was determined by the yellow signal in merged images. WCL: whole-cell lysates.