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Table 2 ChIFN-γ production of splenocytes after ex vivo mitogenic activation

From: Study of the underlying mechanisms and consequences of pathogenicity differences between two in vitro selected G1-H9N2 clones originating from a single isolate

Experiment Group ChIFNg production (OD)
3 dpi 5 dpi
I Negative 0.334 ± 0.124 0.683 ± 0.411
CEF-H9N2 inoculated 0.028 ± 0.002 0.217 ± 0.157
II Negative 0.489 ± 0.422 0.376 ± 0.256
MDCK-H9N2 inoculated 0.012 ± 0.001* 0.017 ± 0.002
  1. Splenocytes were stimulated with PWM (10 µg/mL). Forty-eight hours later, supernatants were harvested and ChIFN-γ production was determined by ChIFN-γ capture ELISA. Data represent the mean ± standard deviation of the OD values (n = 5).
  2. * Significant differences between naïve and infected chickens (P < 0.05).
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Veterinary Research

ISSN: 1297-9716