Experiment
|
Group
|
ChIFNg production (OD)
|
---|
3 dpi
|
5 dpi
|
---|
I
|
Negative
|
0.334 ± 0.124
|
0.683 ± 0.411
|
CEF-H9N2 inoculated
|
0.028 ± 0.002
|
0.217 ± 0.157
|
II
|
Negative
|
0.489 ± 0.422
|
0.376 ± 0.256
|
MDCK-H9N2 inoculated
|
0.012 ± 0.001*
|
0.017 ± 0.002
|
- Splenocytes were stimulated with PWM (10 µg/mL). Forty-eight hours later, supernatants were harvested and ChIFN-γ production was determined by ChIFN-γ capture ELISA. Data represent the mean ± standard deviation of the OD values (n = 5).
- * Significant differences between naïve and infected chickens (P < 0.05).