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Table 1 List of primers used to quantify the relative expression of immune genes by real-time RT-PCR

From: Study of the underlying mechanisms and consequences of pathogenicity differences between two in vitro selected G1-H9N2 clones originating from a single isolate

  Forward primer Reverse primer References
GADPH 5′-GACGTGCAGCAGGAACACTA-3′ 5′-TCTCCATGGTGGTGA AGACA-3′ [67]
IL-6 5′-ATCCGGCAGATGGTGATAAA-3′ 5′-CCCTCACGGTCTTCTCCATA-3′ [67]
IL-1β 5′-GCTCTACATGTCGTGTGTGATGAG-3′ 5′-TGTCGATGTCCCGCATGA-3′ [68]
INF-α 5′-GACATGGCTCCCACACTACC-3′ 5′-AGGCGCTGTAATCGTTGTCT-3′ [67]
INF-β 5′-GCCCACACACTCCAAAACACTG-3′ 5′-TTGATGCTGAGGTGAGCGTTG-3′ [69]
INF-γ 5′-GTGAAGAAGGTGAAAGATATCATGGA-3′ 5′-GCTTTGCGCTGGATTCTCA-3′ [68]
TLR-7 5′-TCTGGACTTCTCTAACAACA-3′ 5′-AATCTCATTCTCATTCATCATCA-3′ [70]
TLR-3 5′-GCTATTGAGCAAAGTCGAGA-3′ 5′-ACAGGGGGCACTTTACTATT-3′ [70]