Study of the underlying mechanisms and consequences of pathogenicity differences between two in vitro selected G1-H9N2 clones originating from a single isolate

Table 1 List of primers used to quantify the relative expression of immune genes by real-time RT-PCR

	Forward primer	Reverse primer	References
GADPH	5′-GACGTGCAGCAGGAACACTA-3′	5′-TCTCCATGGTGGTGA AGACA-3′	[67]
IL-6	5′-ATCCGGCAGATGGTGATAAA-3′	5′-CCCTCACGGTCTTCTCCATA-3′	[67]
IL-1β	5′-GCTCTACATGTCGTGTGTGATGAG-3′	5′-TGTCGATGTCCCGCATGA-3′	[68]
INF-α	5′-GACATGGCTCCCACACTACC-3′	5′-AGGCGCTGTAATCGTTGTCT-3′	[67]
INF-β	5′-GCCCACACACTCCAAAACACTG-3′	5′-TTGATGCTGAGGTGAGCGTTG-3′	[69]
INF-γ	5′-GTGAAGAAGGTGAAAGATATCATGGA-3′	5′-GCTTTGCGCTGGATTCTCA-3′	[68]
TLR-7	5′-TCTGGACTTCTCTAACAACA-3′	5′-AATCTCATTCTCATTCATCATCA-3′	[70]
TLR-3	5′-GCTATTGAGCAAAGTCGAGA-3′	5′-ACAGGGGGCACTTTACTATT-3′	[70]

ISSN: 1297-9716