Figure 8

CXCL13 is a direct target of ssc-miR-296-5p. A Diagram of the predicted targeting site within the 3′-UTR of CXCL13 (3′UTR-wt). The mutated 3′-UTR (3′UTR-mut) contains mutated sequence that is not complementary to the seed sequence (CCGGGAG) of ssc-miR-296-5p. B Expression of ssc-miR-296-5p in PK15 cells at 24 h post-transfection with 800 nM inhibitor (inhi) or negative control (NC). C Luciferase activity of the constructs harboring the wild-type or mutant 3′-UTR of the CXCL13 gene in PK15 cells with 800 nM ssc-miR-296-5p inhibitor or negative control. Data are represented as the mean value ± SE of three experiments. *, **, and *** indicate significance at the P value threshold levels of 0.05, 0.01, and 0.001, respectively.