Figure 5

CXCL13 can suppress the apoptosis of murine splenocytes during PCV2 infection. Splenocytes were freshly isolated from murine spleen and treated with PCV2 at an MOI of 0.1 TCID₅₀ alone or along with CXCL13 (1 μg/mL). The cells were analyzed by flow cytometry for PI (y axis) and FITC-conjugated annexin V (x axis) at 2, 4, 8, 16, and 24 hpi after treatment. The total percentages of PI⁻ annexin V⁺ cells (Q4) and PI⁺ annexin V⁺ cells (Q2) indicate the apoptosis rate. The nine scatter plots shown on the left are from a single experiment, which was representative of three separately performed experiments. The bar graphs shown on the right indicate mean value ± SE of three experiments. * and *** indicate significance at the P value threshold levels of 0.05 and 0.001, respectively.