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Figure 5 | Veterinary Research

Figure 5

From: Suppression of lymphocyte apoptosis in spleen by CXCL13 after porcine circovirus type 2 infection and regulatory mechanism of CXCL13 expression in pigs

Figure 5

CXCL13 can suppress the apoptosis of murine splenocytes during PCV2 infection. Splenocytes were freshly isolated from murine spleen and treated with PCV2 at an MOI of 0.1 TCID50 alone or along with CXCL13 (1 μg/mL). The cells were analyzed by flow cytometry for PI (y axis) and FITC-conjugated annexin V (x axis) at 2, 4, 8, 16, and 24 hpi after treatment. The total percentages of PI annexin V+ cells (Q4) and PI+ annexin V+ cells (Q2) indicate the apoptosis rate. The nine scatter plots shown on the left are from a single experiment, which was representative of three separately performed experiments. The bar graphs shown on the right indicate mean value ± SE of three experiments. * and *** indicate significance at the P value threshold levels of 0.05 and 0.001, respectively.

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