Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout ( Oncorhynchus mykiss )

Table 2 Primers used for immune gene analysis

Gene	Primer sequences	Amplicon length	Reference	Gene Bank No.
Elongation Factor (EF1a)	GATCCAGAAGGAGGTCACCA TTACGTTCGACCTTCCATCC	150	[43]	AF498320.1
Mx	AGCTCAAACGCCTGATGAAG ACCCCACTGAAACACACCTG	142	[43]	NM_001171901
Viperin	ACGACCTCCAGCTCCCAAGT GTCCAGGTGGCTCTTCCTGC	173	[43]	AF076620.1
Interferon type 1	AAAACTGTTTGATGGGAATATGAAA TGTTTCAGTCTCCTCTCAGGTT	141	[43]	NM_001124531
Interferon gamma	CAAACTGAAAGTCCACTATAAGATCTCCA GGTCCAGCCTCTCCCTCAC	188	[44]	FM864345.1
CD-4	CCTGCTCATCCACAGCCTAT CTTCTCCTGGCTGTCTGACC	111	[43]	AY973030.1
CD-8 alpha	AGTCGTGCAAAGTGGGAAAG GGTTGCAATGGCATACAGTG	123	[43]	NM_001124263