|
Group
|
Number of fish
|
Trial length (weeks)
|
Sampling time points (weeks post-challenge-wpc)a
|
Fish sampled per time point
|
Samples
|
Analysis
|
|---|
|
Negative control
|
150 mock injected
|
10
|
2, 4, 6, 8, 10
|
2
|
Blood
|
Antibody detection
|
|
Heart
|
Virus qPCR; CD4 CD8
|
|
Spleen
|
Virus qPCR; IFN
|
|
Organs
|
Histopathology
|
|
150 cohabitants
|
10
|
2, 4, 6, 8, 10
|
2
|
Blood
|
WB—antibody detection—Hct
|
|
Heart
|
Virus qPCR; CD4 CD8
|
|
Spleen
|
Virus qPCR; IFN
|
|
Organs
|
Histopathology
|
|
Purified PRV-3 particles
|
50 purified PRV-3 injected
|
10
|
2, 4, 6, 8, 10
|
6
|
Blood
|
Antibody detection
|
|
Heart
|
Virus qPCR; CD4 CD8
|
|
Spleen
|
Virus qPCR
|
|
Organs
|
Histopathology
|
|
50 cohabitants
|
10
|
2, 4, 6, 8, 10
|
6
|
Blood
|
WB—antibody detection—Hct
|
|
Heart
|
Virus qPCR; CD4 CD8
|
|
Spleen
|
Virus qPCR; IFN
|
|
Organs
|
Histopathology
|
|
Positive control
|
50 PRV-3 infected blood injected
|
10
|
2, 4, 6, 8, 10
|
6
|
Blood
|
Antibody detection
|
|
Heart
|
Virus qPCR; CD4 CD8
|
|
Spleen
|
Virus qPCR
|
|
Organs
|
Histopathology
|
|
50 cohabitants
|
10
|
2, 4, 6, 8, 10
|
6
|
Blood
|
WB—antibody detection—Hct
|
|
Heart
|
Virus qPCR; CD4 CD8
|
|
Spleen
|
Virus qPCR; IFN
|
|
Organs
|
Histopathology
|
- aIn addition 9 fish were collected prior to exposure and investigated for presence of PRV-3 and heart histopathology.
