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Table 1 Design of the experimental trial

From: Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss)

Group

Number of fish

Trial length (weeks)

Sampling time points (weeks post-challenge-wpc)a

Fish sampled per time point

Samples

Analysis

Negative control

150 mock injected

10

2, 4, 6, 8, 10

2

Blood

Antibody detection

Heart

Virus qPCR; CD4 CD8

Spleen

Virus qPCR; IFN

Organs

Histopathology

150 cohabitants

10

2, 4, 6, 8, 10

2

Blood

WB—antibody detection—Hct

Heart

Virus qPCR; CD4 CD8

Spleen

Virus qPCR; IFN

Organs

Histopathology

Purified PRV-3 particles

50 purified PRV-3 injected

10

2, 4, 6, 8, 10

6

Blood

Antibody detection

Heart

Virus qPCR; CD4 CD8

Spleen

Virus qPCR

Organs

Histopathology

50 cohabitants

10

2, 4, 6, 8, 10

6

Blood

WB—antibody detection—Hct

Heart

Virus qPCR; CD4 CD8

Spleen

Virus qPCR; IFN

Organs

Histopathology

Positive control

50 PRV-3 infected blood injected

10

2, 4, 6, 8, 10

6

Blood

Antibody detection

Heart

Virus qPCR; CD4 CD8

Spleen

Virus qPCR

Organs

Histopathology

50 cohabitants

10

2, 4, 6, 8, 10

6

Blood

WB—antibody detection—Hct

Heart

Virus qPCR; CD4 CD8

Spleen

Virus qPCR; IFN

Organs

Histopathology

  1. aIn addition 9 fish were collected prior to exposure and investigated for presence of PRV-3 and heart histopathology.