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Table 1 Design of the experimental trial

From: Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss)

Group Number of fish Trial length (weeks) Sampling time points (weeks post-challenge-wpc)a Fish sampled per time point Samples Analysis
Negative control 150 mock injected 10 2, 4, 6, 8, 10 2 Blood Antibody detection
Heart Virus qPCR; CD4 CD8
Spleen Virus qPCR; IFN
Organs Histopathology
150 cohabitants 10 2, 4, 6, 8, 10 2 Blood WB—antibody detection—Hct
Heart Virus qPCR; CD4 CD8
Spleen Virus qPCR; IFN
Organs Histopathology
Purified PRV-3 particles 50 purified PRV-3 injected 10 2, 4, 6, 8, 10 6 Blood Antibody detection
Heart Virus qPCR; CD4 CD8
Spleen Virus qPCR
Organs Histopathology
50 cohabitants 10 2, 4, 6, 8, 10 6 Blood WB—antibody detection—Hct
Heart Virus qPCR; CD4 CD8
Spleen Virus qPCR; IFN
Organs Histopathology
Positive control 50 PRV-3 infected blood injected 10 2, 4, 6, 8, 10 6 Blood Antibody detection
Heart Virus qPCR; CD4 CD8
Spleen Virus qPCR
Organs Histopathology
50 cohabitants 10 2, 4, 6, 8, 10 6 Blood WB—antibody detection—Hct
Heart Virus qPCR; CD4 CD8
Spleen Virus qPCR; IFN
Organs Histopathology
  1. aIn addition 9 fish were collected prior to exposure and investigated for presence of PRV-3 and heart histopathology.