Figure 8
From: Gammaherpesvirus BoHV-4 infects bovine respiratory epithelial cells mainly at the basolateral side
BoHV-4 binds to nasal and tracheal epithelial cells at apical and basolateral surfaces treated with PBS or EGTA. BREC were inoculated at 4 °C for 1 h with BoHV-4 particles (MOI = 10). Representative confocal images of nasal (A) and tracheal epithelial cells (B) with bound BoHV-4 particles (green), cell nuclei are stained in blue. The scale bar represents 20 μm. The percentage of cells with bound virus particles was calculated based on 10 random fields of 300 cells from nasal (C) and tracheal epithelial cells (D). Three independent experiments were performed and data are given as means + SD. Significant (p < 0.05) differences are indicated with an asterisk.