Figure 6

Nasal epithelial cells were inoculated with BoHV-4 (MOI = 2) at either the apical surface or basolateral surface after treatment with PBS or EGTA. Representative confocal images of BoHV-4 (red) infection in nasal epithelial cells (green) after treatment with PBS or EGTA at 24 h, 48 h and 72 hpi; nuclei were stained with Hoechst (blue) (A). The scale bar represents 50 μm. The percentage of BoHV-4 infection was counted in 10 different fields of nasal epithelial cells after treatment with PBS or EGTA (B). In addition, the virus titers in culture medium were determined (C). Data are given as means + SD of triplicate independent experiments. These lower-case letters explain the p value. Significant differences (p < 0.05) are presented by the use of different letters.