Table 1 Primers and annealing temperature for real-time PCR.
From: Role of p53 in pseudorabies virus replication, pathogenicity, and host immune responses
Genes | Primer sequence (5′-3′) | Annealing (°C) |
|---|---|---|
gB | (F)CGGCATCGCCAACTTCTTC (R)GTCCTCCTTGAGCGTCTTCGT | 61 |
IL-6 | (F)AGTCCGGAGAGGAGACTTCA (R)ATTTCCACGATTTCCCAGAG | 60 |
TNF-α | (F)GGGACAGTGACCTGGACTGT (R)GCTCCAGTGAATTCGGAAAG | 61 |
Oasl2 | (F)ACAATTTCCAAAACGAGGTC (R)TTCCCATCCCTTTCTTCTTC | 59 |
Ifi44 | (F)GACAGATACCAGTTCGATTC (R)TTTTCTTGATCTTTGCCACC | 60 |
Usp18 | (F)CAAGGAACAGTCTGAAATACAC (R)CACAGTAATGACCAAAGTCAG | 60 |
Ifit1 | (F)AGAACAGCTACCACCTTTAC (R)TTCTTGATGTCAAGGAACTG | 61 |
Ly6a | (F)GAGAGGAAGTTTTATCTGTGC (R)TCTCAAATGGGACTCCATAG | 60 |
Gbp10 | (F)CTAACCGGAAGTGTTTTGTC (R)CAGAATCCCTAGTTTATTCCC | 59 |
Gbp4 | (F)AGCTAACGAAGGAACAAAAG (R)GATGTTATGTCCCAGTTGATG | 60 |
Gbp3 | (F)CTGTTCGAGATTTTGCTCTG (R)TGGACTTTGAGATTGTCTCC | 60 |
Gbp7 | (F)GAGTGAAGGCAAATCATGTC (R)CTGTTTCTGTCTTAGTAGCTC | 60 |
Stat1 | (F)TTTGACAGTATGATGAGCAC (R)AGCAAATGTGATGCTCTTTC | 61 |
Ddx58 | (F)GAGAGTCACGGGACCCACT (R)CGGTCTTAGCATCTCCAACG | 61 |
Ifih1 | (F)TGATGCACTATTCAAGAACTAACA (R)TCTGTGAGACGAGTTAGCCAAG | 59 |
Ifitm3 | (F)TCATCATTGTTCTTAACGCTCA (R)CGGAAGTCGGAATCCTCTAT | 60 |
Mx1 | (F)GAAGGCAAGGTCTTGGATG (R)GCTGACCTCTGCACTTGACT | 60 |
Ifi44 | (F)TTCAACTCAGTGGAAGTCTGCT (R)GGAGTGTTTCCCCGCTTTTTC | 61 |
Ccl2 | (F)TCTGTGCTGACCCCAAGAAGG (R)TGGTTGTGGAAAAGGTAGTGGAT | 59 |
Cxcl10 | (F)TCCCTCTCGCAAGGAC (R)TTGGCTAAACGCTTTCAT | 59 |
β-actin | (F)AGGTGACAGCATTGCTTCTG (R)GCTGCCTCAACACCTCAAC | 60 |