Figure 4
From: Role of p53 in pseudorabies virus replication, pathogenicity, and host immune responses
IL-6 and TNF-α mRNA and antibody levels were detected in the brains of the p53−/− and wild-type mice following intracranial inoculation. Four- to 8-week-old male p53−/− (n = 6) and wild-type (n = 6) mice were inoculated with 105 TCID50 of PRV (Bartha) intracranially. At 6 days post-infection, the brains of the infected mice were harvested (A–D). IL-6 and TNF-α mRNA and antibody levels were detected by relative quantitative real-time PCR and ELISA. Each data point is the level of IL-6 and TNF-α mRNA or antibodies in the brain of one mouse. The horizontal bars indicate the mean for each group. *P < 0.05; **P < 0.01.