Transmission studies of chronic wasting disease to transgenic mice overexpressing human prion protein using the RT-QuIC assay

Table 1 Tg66 and tgRM mice inoculated with CWD

Mouse strain	Inoculum	Number of mice^a	Number of mice excluded^b	Days post-inoculation until euthanasia (range, group average)	Clinical suspects^c
Tg66	Elk-2	17	2	600–719, 660	7/17
Tg66	MD-1	19	0	467–719, 631	2/19
Tg66	WTD-1	16	1	503–744, 685	6/16
TgRM	Elk-2	19	1	538–748, 706	1/19
TgRM	MD-1	15	5	551–748, 692	0/15
TgRM	WTD-1	11	2	496–798, 696	2/11

^aIncludes the total number of mice inoculated and observed for > 450 days.
^bIndicates the number of mice that were inoculated but euthanized prior to 450 days and have been excluded from PrP screening tests with the exception of three tgRM mice that were euthanized at 369, 371 and 432 dpi and tested by RT-QuIC.
^cClinical suspects in the tg66 mice included mice that displayed one or more of the following signs: gait abnormalities, hind limb weakness, tremors, kyphosis or weight loss. Clinical suspects in the tgRM mice included two mice with abnormal gait and one thin mouse.

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