Figure 6

The activation of MAPK and NF-κB signaling pathways is mediated by Syk. A Detection of p38, JNK, ERK1/2, and NF-κB expression in OREC after mannan stimulation and R406 blockade by qPCR. The relative mRNA abundance was calculated using the 2^−ΔΔCt method relative to β-actin. B Protein amounts are represented by the value shown in gray for the target protein/β-actin. Statistical analyses were performed using the ImageJ software. Data are mean ± SD (n = 3). Different letters indicate significantly different means (P < 0.01).