Figure 4

Mannan-stimulated effect on Syk in OREC. A Mannan stimulates Syk expression in OREC, as measured by qPCR; the relative mRNA abundance was calculated using the 2^−ΔΔCt method relative to β-actin. Data are mean ± SD (n = 3). *P < 0.05, **P < 0.01 vs. the unstimulated group. B Mannan stimulates Syk expression in OREC, as measured by Western blotting. Protein amounts are represented by the value shown in gray for Syk/β-actin. Statistical analyses were performed using the ImageJ software. Data are mean ± SD (n = 3). *P < 0.05, **P < 0.01 vs. the unstimulated group. C Expression of Syk and p-Syk was determined by Western blotting at 0, 5, 15, 30, 45, and 60 min after incubation with mannan. Data are mean ± SD (n = 3). Different letters indicate significantly different means (P < 0.01).