Figure 2

Mannan induces SBD-1 expression via Dectin-2. A Mannan stimulates Dectin-2 expression in OREC as measured by qPCR; the relative mRNA abundance was calculated using the 2^−ΔΔCt method relative to β-actin. Data are mean ± SD (n = 3). *P < 0.05, **P < 0.01 vs. the unstimulated group. B Mannan stimulates Dectin-2 expression in OREC as measured by Western blotting. Protein amounts are represented by the value shown in gray for the Dectin-2 protein/β-actin. Statistical analyses were performed using the ImageJ software. Data are mean ± SD (n = 3). *P < 0.05, **P < 0.01 vs. the unstimulated group. C The SBD-1 expression was determined by qPCR. OREC were incubated with Dectin-2 Mouse mAb (0.1, 1, 10 μg/mL) for 30 min prior to the addition of 50 μg/mL mannan for 4 h. Dectin-2 blockade by Dectin-2 Mouse mAb reduces mannan-induced SBD-1 expression in OREC. SBD-1 mRNA abundance was calculated using the 2^−ΔΔCt method relative to β-actin. Data are mean ± SD (n = 3). Different letters indicate significantly different means (P < 0.01).