Figure 5
From: Effect of anacardic acid against echinococcosis through inhibition of VEGF-induced angiogenesis
The cytotoxicity and mechanism of AA against Echinococcus. A Cytotoxicity of AA was measured via Chang liver cell line and HepG2 cell line by MTT assay. B The EC50 value of AA on protoscolices and the IC50 values of AA on HepG2 cells and Chang liver cells were calculated in OriginPro 8. C Images of blood vessel of metacestodes in different treatment groups. D The sections of the metacestodes from different treatment groups were showed by IHC analysis with anti CD34 antibodies. E The mean density of CD34 in different treatment groups were showed, which was calculated by Image-J software. F The level of VEGF was measured after treatment with ABZ and AA by ELISA. G Schematic representation of the mechanism underlying the AA-mediated inhibition of VEGF-induced angiogenesis.