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Figure 5 | Veterinary Research

Figure 5

From: Oral vaccination with Trichinella spiralis DNase II DNA vaccine delivered by attenuated Salmonella induces a protective immunity in BALB/c mice

Figure 5

Recognition of native TsDNase II at the cuticle of various T. spiralis phases by IIFT with serum from mice vaccinated with TsDNase II vaccine, pcDNA3.1 alone or PBS. The intensive fluorescence staining at the external cuticle of IIL at 15, 18 and 24 hpi and adult worms at 3 and 5 dpi was detected by using anti-TsDNase II immune serum, but not by serum from only pcDNA3.1 or PBS control mice. No staining was seen on the cuticle of the ML and IIL at 2, 4, 6, 12 hpi by using anti-TsDNase II immune serum. The results indicate that native TsDNase II is present at the worm cuticle only after the 2nd molting of this nematode. Besides, immunostaining was observed at the cuticle of all various T. spiralis phases using infection serum as positive control. Scale bar = 50 μm.

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