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Figure 2 | Veterinary Research

Figure 2

From: Oral vaccination with Trichinella spiralis DNase II DNA vaccine delivered by attenuated Salmonella induces a protective immunity in BALB/c mice

Figure 2

Transcription and expression of recombinant pcDNA3.1-TsDNase II in the MLN and spleen of vaccinated mice. Left: Transcriptions of TsDNase II in spleen and MLN of vaccinated mice were analyzed by RT-PCR. TsDNase II amplified fragments were observed in spleen (lane 1) and MLN (lane 2) from TsDNase II-vaccinated mice, but neither in spleen (lane 3) and MLN (lane 4) from pcDNA3.1 control mice, nor in spleen (lane 5) and MLN (lane 6) from the PBS group. Right: IIFT with anti-TsDNase II serum show that the fluorescence staining was detected in MLN (A) and spleen (D) of vaccinated mice, but the section of MLN (B) and spleen (E) of vaccinated mice were probed by normal serum. No immunostaining in MLN (C) and spleen (F) of pcDNA3.1 control mice was detected by anti-rTsDNase II serum. Scale bar: 50 μm.

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Veterinary Research

ISSN: 1297-9716