Table 1 Monoclonal anti-PrP antibodies used in this study
Clone | Epitope location on bovine PrP | Species reactivity | Vendor | Dilution |
|---|---|---|---|---|
P4 | 101–107 | Cattle, sheep | R-Biopharm, Darmstadt, Germany | 1: 5000 (WB) |
6H4 | 156–163 | Cattle, sheep, mice | Prionics, Schlieren, Switzerland | 1: 5000 (WB) |
SAF84 | 175–180 | Cattle, sheep, mice | Bertin Pharma, Montigny le Bretonneux, France | 1: 5000 (WB) |
31C6 | 155–163 | Cattle, sheep, mice | Frontier Science, Hokkaido, Japan | 1: 1800 (IHC) |
T1 | 149–155 | Cattle, sheep, mice | Not for sale (See reference number 41.) | 1: 4500 (IHC) |