Neuroanatomical changes and PrPd accumulation in the brains of TgBoPrP mice infected with Sh294 and L-BSE. Vacuolation in the following brain regions was scored on a scale of 0–5 A: 1, dorsal medulla; 2, cerebellar cortex; 3, superior cortex; 4, hypothalamus; 5, thalamus; 6, hippocampus; 7, septal nuclei of the paraterminal body; 8, cerebral cortex at the level of the hypothalamus and thalamus; 9, cerebral cortex ash the level of the septal nuclei of the paraterminal body. Results are shown as the mean ± standard deviation (circles, Sh294; diamonds, L-BSE). Sections were subjected to H&E staining. Representative examples of vacuolation in the hippocampus B, C, E, G, cortex D, F, thalamus H, J, and cerebellum I, K are shown. Representative PrPd immunohistochemistry in the hippocampus is shown L, M. Scale bars indicate 200 µm B, C, L, M and 50 µm D–K.