Biochemical analysis of PrPres from Sh294 in TgBoPrP mice. PrPres banding patterns were compared in TgBoPrP mice infected with Sh294 and BSEs using the mAbs 6H4 A and SAF84 B. PrPres was also characterized using the mAbs P4 C and SAF84 D after PNGase deglycosylation. Lane 1, Sh294; lane 2, L-BSE; lane 3, C-BSE; lane 4, H-BSE. Brain homogenates from sheep affected with classical scrapie isolate (lane Cl in B) and CH1641-like scrapie isolate (Sh294: lane Ch in B, D) were also loaded for comparison. Lanes from 1 to 4 in A, B were loaded with 0.6, 0.06, 0.5, and 0.4 mg brain equivalent, respectively. Lanes Cl and Ch in B were loaded with 0.3 and 2.0 mg brain equivalent, respectively. Lanes from 1 to 4 in C were loaded with 0.03, 0.006, 0.02, and 0.02 mg brain equivalent, respectively. Lanes from left to right in D were loaded with 0.2, 0.2, 0.06, 0.05 and 0.02 mg brain equivalent, respectively. Molecular markers are shown on the left of each panel. Glycoform profiles of PrPres in brains of TgBoPrP mice infected with Sh294, C-BSE, and L-BSE are given E. PrPres was detected with mAb 6H4. Symbols: red squares, Sh294; blue triangles, C-BSE; green circles, L-BSE.