Stimulation of different TLR2 receptors by three TLR2 agonists. A All stable HEK293T cell lines express comparable amounts of FLAG-tagged TLR2, as detected by anti-FLAG staining using flow cytometry. One representative experiment is shown (B–D). All stable HEK293T-TLR2 expressing cell lines were stimulated with different concentrations of the TLR2 agonists MALP-2 (B), Pam2CSK4 (C), and Pam3CSK4 (D) for 6 h, after which supernatant was harvested and IL-8 production within the supernatant was determined using an anti-IL8 ELISA. The data shown represents the relative IL-8 production (IL-8 production was normalized to the highest agonist concentrations for human TLR2). The mean ± standard deviation (SD) of at least three independent experiments is shown.