Figure 3

Gene silencing in Psoroptes ovis by RNAi. Mean expression levels of transcripts encoding Pso o 2, PoGST-mu1 and Poβtub normalised to Poβactin (copies/µL) determined by qPCR compared to control mites immersed in dsRNA encoding lacZ are shown for adult male mites immersed overnight at 4 °C in dsRNA representing Pso o 2 (A), PoGST-mu1 (C) and Poβtub (E), or immersed in dsRNA representing Pso o 2 (B), PoGST-mu1 (D) and Poβtub (F) overnight at 4 °C followed by 48 h in a humidity incubator (25 °C, 75% RH). n = 5 for each treatment group with the exception of the lacZ control group in the Poβtub overnight incubation only experiment where n = 4, error bars indicate mean ± SEM.