Small ruminant lentivirus infection influences expression of acute phase proteins and cathelicidin genes in milk somatic cells and peripheral blood leukocytes of dairy goats

Reczyńska, Daria; Zalewska, Magdalena; Czopowicz, Michał; Kaba, Jarosław; Zwierzchowski, Lech; Bagnicka, Emilia

doi:10.1186/s13567-018-0607-x

Veterinary Research

Table 1 Primer sequences, position in gene, accession number in GenBank, and product sizes used in qPCR

From: Small ruminant lentivirus infection influences expression of acute phase proteins and cathelicidin genes in milk somatic cells and peripheral blood leukocytes of dairy goats

Gene name	Gene symbol	Primer sequence		Product size (bp)	GenBank/UniProt assession^a	References
Serum amyloid A3 protein	SAA	F	CTGGGCTGCTAAAGTGATCAGTAAC	69	EU884570.1	[59]
Serum amyloid A3 protein	SAA	R	CCCTTGAGCAGAGGGTCTGTGATT	69	EU884570.1	[59]
Haptoglobin	Hp	F	TAATGCCCATCTGCCTAC	162	XM_005692202.3	[60]
Haptoglobin	Hp	R	CGCCCTCATAGTGTTTCA	162	XM_005692202.3	[60]
C-reactive protein	CRP	F	CTGGCTTGGGAGATTG	134	XM_018046353.1	[60]
C-reactive protein	CRP	R	AGTGAGGGTAAGGGATT	134	XM_018046353.1	[60]
α-lactalbumin	LLBA	F	TGACATTTGTGTGTGCCAAGA	198	NM_001285635.1	PRIMER3^b
α-lactalbumin	LLBA	R	CAAGGGGGTACAAAGAAGCA	198	NM_001285635.1	PRIMER3^b
α-acid glycoprotein	AGP	F	TTGCTTGGCTGCAGGTGT	197	XM_012152252.2	[11]
α-acid glycoprotein	AGP	R	CAATGGTCTGGTACTCTCTCTG	197	XM_012152252.2	[11]
Ceruloplasmin	Cp	F	GAGCATGAAGGGGCCATTTATC	130	NM_001256556.1	[61]
Ceruloplasmin	Cp	R	GCTGTCTTCCTCACCAGG	130	NM_001256556.1	[61]
Fibrinogen α chain	Fbα	F	TGAGATCCTGAGGCGCAAAG	104	NM_001033626.1	[61]
Fibrinogen α chain	Fbα	R	TGTCCACCTCCAATCGTTTCAT	104	NM_001033626.1	[61]
Fibrinogen β chain	Fbβ	F	GACAACGACGGCTGGAAAAC	124	NM_001142917	[61]
Fibrinogen β chain	Fbβ	R	ACGCTCCACCCCAGTAGTAT	124	NM_001142917	[61]
Fibrinogen ɣ	Fbɣ	F	TGCCAATAAGGGGGCCAAAG	134	NM_173911	[61]
Fibrinogen ɣ	Fbɣ	R	GACTGCCATCAAGCCTCTTCT	134	NM_173911	[61]
Bactenecin-5; cathelicidin-2	BAC5	F	GTGGAATTCACGGTGAAGGAGAC	390	Y18873.1	[52]
Bactenecin-5; cathelicidin-2	BAC5	R	CTCAGGCCAAATGAGAT	390	Y18873.1	[52]
Bactenecin-7.5; cathelicidin-3	BAC7.5	F	GTGGAATTCACGGTGAAGGAGAC	383	AJ243125.1	[52]
Bactenecin-7.5; cathelicidin-3	BAC7.5	R	AGTGCTAACCTTGATGTT	383	AJ243125.1	[52]
Cathelicidin-6	MAP28	F	GTGGAATTCACGGTGAAGGAGAG	225	AJ243126.1	[52]
Cathelicidin-6	MAP28	R	AATTGGGCCGACTTTGTGCC	225	AJ243126.1	[52]
Cathelicidin-7	MAP34	F	ACCGAATTCAGCTACAGGGAGGCCGT	428	AJ243127.1	[52]
Cathelicidin-7	MAP34	R	ACCTGATCCTTAGGACTTC	428	AJ243127.1	[52]

bp: base pairs, F: forward primer, R: reverse primer.
^aNCBI [62].
^bPRIMER3 [63].

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ISSN: 1297-9716

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