Table 2 Oligonucleotide primers used in this study
From: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2
Primer name | Sequence (5′-3′) | Construct |
|---|---|---|
zmp-ID1 | CCTTGGTTTAGATGCCC | p4Δzmp |
zmp-ID2 | CTGAGACAAGTCCCACT | p4Δzmp |
zmp-ID3 | CAAGCCTTGATGAACTCTAC | p4Δzmp |
zmp-ID4 | GCTATTCCTAGCTTCTACCT | p4Δzmp |
zmp-ID5 | TGTTTCTTTCATCCTTGACAG | p4Δzmp |
zmp-ID6 | CTTGTTAATGATTATTTAATAAGTTGTTACTCCCTAAAATAG | p4Δzmp |
zmp-ID7 | TAGGGAGTAACAACTTATTAAATAATCATTAACAAGTTGGTC | p4Δzmp |
zmp-ID8 | ATCTGGCTCATCCATGAC | p4Δzmp |
pMX1-cpec-F | AAATAGTATAGAAACGGCATGCAAGCTTGG | pMXzmp |
pMX1-cpec-R | TCGGAGGTCCTTTAGCCCGGGTACCGAGCT | pMXzmp |
zmp-cpec-F | CCAAGCTTGCATGCCGTTTCTATACTATTT | pMXzmp |
zmp-cpec-R | AGCTCGGTACCCGGGCTAAAGGACCTCCGA | pMXzmp |
pET101-zmp-F | CACCATGGCTCGATATAACCATGCAATC | pET101zmp |
pET101-zmp-R | TGGGTTAAAAATCGATGTTCTG | pET101zmp |