Figure 3
From: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2
S. suis does not activate matrix metalloprotease-9 (MMP-9). Pro-enzyme MMP-9 was incubated for 1 h with purified recombinant Zmp protein (100 µg), washed bacteria (bact) or bacterial supernatant (sup) from S. suis (SS) or S. pneumoniae (SP). Samples were separated in non-denaturing conditions on a gelatin B zymogram. Coomassie coloration shows a second gelatinolytic band for washed S. pneumoniae bacteria or bacterial supernatants (lane 2 and 3) corresponding to activated MMP-9, while no second band was observed for media (lane 1) washed S. suis bacteria (lane 4), bacterial supernatant (10X, lane 5) or purified Zmp protein (lane 6).