Table 1 Experimental design for trials to assess alternate infection routes
From: Alternate routes of influenza A virus infection in Mallard (Anas platyrhynchos)
Experiment | Trial | # Ducks | Age of ducks (months) | Mode of inoculation | Housing |
|---|---|---|---|---|---|
Cloacal drinking | Cloacal inoculation | 5 | 4 | Inoculation of 2 mL virus into the cloaca with canula | Each duck in a separate cage immediately following inoculation |
Cloacal inoculation | 5 | 6 | Inoculation of 2 mL virus into the cloaca with canula | Each duck in a separate cage immediately following inoculation | |
Cloacal exposure | 4 | 5 | Placed in virus laden water (6 mL virus stock + 2 L water) in specially designed inoculation box | Each duck in a separate cage immediately following inoculation | |
Preening | Preening cages | 5 | 6 | Bathed with virus laden water (3 mL virus stock + 1 L water) | Each duck in a separate cage immediately following inoculation |
Preening transmission (inoculation) | 5 | 6 | Bathed with virus laden water (3 mL virus stock + 1 L water) | Ducks placed in an experimental room with conspecifics | |
Preening transmission (contacts) | 5 | 6 | Unexposed contacts | Ducks placed in an experimental room with conspecifics |