Figure 1
From: The adjuvant G3 promotes a Th1 polarizing innate immune response in equine PBMC
Relative expression of the genes encoding IFN-γ, IL-1β, IL-6, IL-8, IL-12p40 and IL-23p19 in eqPBMC. PBMC collected from eight horses were cultured in various concentrations of G3 (1, 3 or 5 µg/mL) or in plain growth medium for 18 h. The cytokine gene expression was normalized to the geometric mean for the reference genes (SDHA and RPL32) and calibrated to that in the medium control.