Microscopic lesions in brain of lambs naturally infected with N. caninum. A Focus of gliosis (arrowhead) at the cerebral cortex. HE. 4×. B Diffuse congestion, mainly seen at the white matter of the corona radiata and a focus of necrosis with peripheral gliosis. HE. 4×. C Tissue cyst of N. caninum containing structures compatible with bradyzoites. Note the thick membrane of the cyst. HE. 40×. D Positive labelling of N. caninum tissue cyst. IHC. 40×. All pictures come from lamb offspring of G0 ewes.