Table 1 List of primary and secondary antibodies used for immunohistochemistry
Antibody name | Antigen | Isotype | Supplier | Working concentration |
|---|---|---|---|---|
Rabbit anti-GFP Alexa Fluor® 488 | eGFP transgene | IgG | Thermo Fischer Scientific | 4 µg/mL |
Mouse anti-chicken MHCII (clone TAP1) | Class II MHC | IgG2a | DSHB | 0.2 µg/mL |
Mouse anti-chicken CD11 (clone 8F2) | CD11 | IgG1 | Kind gift from Dr Sonja Hartle (LMU) | 3 µg/mL |
Mouse anti-chicken TIM4 (clone JH9) | TIM4 | IgG1 | In house [27] | 2 µg/mL |
Mouse anti-chicken LEP100 | Chicken lysosomal-associated membrane glycoprotein (LAMP1), present in late endosome and lysosomes | IgG1 | DSHB | 10 µg/mL |
Mouse anti-chicken IgG (clone G-1) | IgY | IgG1 | Southern biotech | 5 µg/mL |
Mouse anti-chicken Bu-1 (clone AV20) | chB6 | IgG1 | Southern biotech | 5 µg/mL |
Mouse anti-chicken CD3 (clone CT3) | CD3 | IgG1 | Southern biotech | 5 µg/mL |
Jacalin-biotin | O-glycosidically linked oligosaccharides | – | Vector Laboratories | 10 µg/mL |
Phalloidin-Alexa Fluor®647 | F-actin | – | Thermo Fischer Scientific | 0.132 µm |
Mouse IgG1 (NC-1390-P) | IgG1 isotype | IgG1 | Thermo Fischer Scientific | 5 µg/mL |
Mouse IgG2a (NC-1391-P) | IgG2a isotype | IgG2a | Thermo Fischer Scientific | 5 µg/mL |
Donkey anti-rabbit IgG Alexa Fluor® 488 | Rabbit IgG | IgG | Invitrogen | 2 µg/mL |
Goat anti-mouse IgG1 Alexa Fluor® 568 | Mouse IgG1 | IgG1 | Invitrogen | 2 µg/mL |
Goat anti-mouse IgG2a Alexa Fluor® 568 | Mouse IgG2a | IgG2a | Invitrogen | 2 µg/mL |
Streptavidin- Alexa Fluor® 568 | Biotin | – | Biolegend | 0.2 µg/mL |