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Table 1 List of primary and secondary antibodies used for immunohistochemistry

From: Visualisation and characterisation of mononuclear phagocytes in the chicken respiratory tract using CSF1R-transgenic chickens

Antibody name Antigen Isotype Supplier Working concentration
Rabbit anti-GFP Alexa Fluor® 488 eGFP transgene IgG Thermo Fischer Scientific 4 µg/mL
Mouse anti-chicken MHCII (clone TAP1) Class II MHC IgG2a DSHB 0.2 µg/mL
Mouse anti-chicken CD11 (clone 8F2) CD11 IgG1 Kind gift from Dr Sonja Hartle (LMU) 3 µg/mL
Mouse anti-chicken TIM4 (clone JH9) TIM4 IgG1 In house [27] 2 µg/mL
Mouse anti-chicken LEP100 Chicken lysosomal-associated membrane glycoprotein (LAMP1), present in late endosome and lysosomes IgG1 DSHB 10 µg/mL
Mouse anti-chicken IgG (clone G-1) IgY IgG1 Southern biotech 5 µg/mL
Mouse anti-chicken Bu-1 (clone AV20) chB6 IgG1 Southern biotech 5 µg/mL
Mouse anti-chicken CD3 (clone CT3) CD3 IgG1 Southern biotech 5 µg/mL
Jacalin-biotin O-glycosidically linked oligosaccharides Vector Laboratories 10 µg/mL
Phalloidin-Alexa Fluor®647 F-actin Thermo Fischer Scientific 0.132 µm
Mouse IgG1 (NC-1390-P) IgG1 isotype IgG1 Thermo Fischer Scientific 5 µg/mL
Mouse IgG2a (NC-1391-P) IgG2a isotype IgG2a Thermo Fischer Scientific 5 µg/mL
Donkey anti-rabbit IgG Alexa Fluor® 488 Rabbit IgG IgG Invitrogen 2 µg/mL
Goat anti-mouse IgG1 Alexa Fluor® 568 Mouse IgG1 IgG1 Invitrogen 2 µg/mL
Goat anti-mouse IgG2a Alexa Fluor® 568 Mouse IgG2a IgG2a Invitrogen 2 µg/mL
Streptavidin- Alexa Fluor® 568 Biotin Biolegend 0.2 µg/mL
  1. DSHB: Developmental Studies Hybridoma Bank; LMU: Ludwig-Maximilian University, Munich, Germany.