PD-1+ T cells were counted with FCM. Splenocytes from experimental mice were stained with PerCP-eFluor710-conjugated anti-CD3, PE-conjugated anti-CD4, APC-conjugated anti-CD8 and FITC-conjugated anti-PD-1. CD3+PD-1+ T cells, CD3+CD4+PD-1+ and CD3+CD8+PD-1+ T cells were detected via FCM. The representative results were depicted (A). The ratio of CD3+PD-1+ T cells in CD3+ T cells (B), the ratio of CD3+CD4+PD-1+ T cells in CD3+ T cells (C) and the ratio of CD3+CD8+PD-1+ T cells in CD3+ T cells (D) are shown respectively. Data collected from five mice per group were expressed as mean ± SD.